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Simms Research Tools

Peptide Reconstitution Calculator

A free peptide calculator, down to the volume. Enter vial mass, diluent volume, and a target amount to get concentration, sample volume, and the U-100 syringe marking.

For laboratory research only

Your inputs

Start with the vial and your research protocol.

Total peptide mass in this vial, in milligrams.

Assumes the final solution volume equals the volume added.

Target amount unit

Amount of peptide in one research sample. 1 mg = 1,000 mcg.

Updates as you typeView result ↓

Your calculation

Ready

U-100 syringe marking

—units

— mL / — µL

Syringe capacity U-100 scale

Schematic only. Check the graduations on your device.

Concentration
— mg/mL
Full samples per vial
—

— mcg in each — mL sample

Starting values are an example, not a recommended amount. Results are rounded for display; sample counts exclude handling losses.

Behind the calculation

Clear inputs.
Transparent math.

Understand your vial’s COA

Find the concentration

Peptide in vial (mg) ÷ solution volume (mL)

Enter your values above.

Convert amount to volume

Target amount (mg) ÷ concentration (mg/mL)

Enter your values above.

Read the U-100 equivalent

Sample volume (mL) × 100 = U-100 markings

Enter your values above.

A little more clarity

Common questions

What does “U-100 units” mean here?

It is a volume scale: 100 markings correspond to 1 mL, so one marking corresponds to 0.01 mL. It does not describe peptide potency or biological activity. The amount of peptide at a marking depends on your calculated concentration. This calculator does not support U-40 or U-500 scales.

Does changing syringe size change the result?

No. The concentration, sample volume, and U-100 equivalent stay the same. Selecting a capacity changes the diagram and checks whether the full sample volume fits in that syringe. Graduation intervals vary by device.

What assumptions does the calculator make?

It assumes complete dissolution and a uniform solution, uses the peptide mass you enter without purity or salt corrections, and treats the added diluent volume as the final solution volume. It does not account for displacement, dead space, or handling losses. Use measured final volume when your protocol requires it.

Can I use this for a peptide blend?

Only if your vial mass and target amount refer to the same basis. Entering the total mass of a blend calculates the combined amount, not the amount of each component. Individual component calculations require that component’s mass in the vial.

Research supplies

Research Water

You’ll need this to reconstitute your peptides. 10mL, shipped from the US.

Shop Research Water