Simms Research Tools
Peptide Reconstitution Calculator
A free peptide calculator, down to the volume. Enter vial mass, diluent volume, and a target amount to get concentration, sample volume, and the U-100 syringe marking.
For laboratory research onlyYour calculation
ReadyU-100 syringe marking
—units
— mL / — µL
Schematic only. Check the graduations on your device.
- Concentration
- — mg/mL
- Full samples per vial
- —
— mcg in each — mL sample
Starting values are an example, not a recommended amount. Results are rounded for display; sample counts exclude handling losses.
Using the calculator
How to use a peptide reconstitution calculator
A calculator for reconstituting peptides needs three values: the total peptide mass in the vial, the final solution volume, and the target amount from the research protocol. Enter them above to calculate mL, µL, and U-100 units.
This peptide mixing calculator performs mass-to-volume conversion only. It does not choose a solvent, preparation method, or target amount.
Enter the vial mass
Use the total peptide mass shown on the vial, in milligrams.
Enter the final volume
Use the final solution volume in milliliters.
Enter the target amount
Choose mcg or mg to match the amount in the research protocol.
Reference table
Peptide reconstitution calculator examples
These examples use a 2 mL final volume. Replace any value in the calculator above to reconstitute peptides at another concentration.
A 5 mg vial at a 2 mL final volume has a concentration of 2.5 mg/mL. A 250 mcg sample occupies 0.1 mL, or 10 units on a U-100 scale.
| Vial mass | 250 mcg | 500 mcg | 1 mg |
|---|---|---|---|
| 5 mg | 10 units | 20 units | 40 units |
| 10 mg | 5 units | 10 units | 20 units |
| 15 mg | 3.33 units | 6.67 units | 13.33 units |
| 20 mg | 2.5 units | 5 units | 10 units |
| 30 mg | 1.67 units | 3.33 units | 6.67 units |
U-100 units are a volume scale: 100 units equals 1 mL. Values are rounded to two decimals.
Compound presets
Dedicated compound calculators
Open a calculator already set to the compound and available vial sizes.
Find the concentration
Peptide in vial (mg) ÷ solution volume (mL)
Enter your values above.
Convert amount to volume
Target amount (mg) ÷ concentration (mg/mL)
Enter your values above.
Read the U-100 equivalent
Sample volume (mL) × 100 = U-100 markings
Enter your values above.
A little more clarity
Common questions
What does “U-100 units” mean here?
It is a volume scale: 100 markings correspond to 1 mL, so one marking corresponds to 0.01 mL. It does not describe peptide potency or biological activity. The amount of peptide at a marking depends on your calculated concentration. This calculator does not support U-40 or U-500 scales.
Does changing syringe size change the result?
No. The concentration, sample volume, and U-100 equivalent stay the same. Selecting a capacity changes the diagram and checks whether the full sample volume fits in that syringe. Graduation intervals vary by device.
What assumptions does the calculator make?
It assumes complete dissolution and a uniform solution, uses the peptide mass you enter without purity or salt corrections, and treats the added diluent volume as the final solution volume. It does not account for displacement, dead space, or handling losses. Use measured final volume when your protocol requires it.
Can I use this for a peptide blend?
Only if your vial mass and target amount refer to the same basis. Entering the total mass of a blend calculates the combined amount, not the amount of each component. Individual component calculations require that component’s mass in the vial.
Is this a peptide mixing calculator?
Yes. It converts the vial mass, final solution volume, and target amount entered by the researcher. It does not select a solvent or preparation method.
Does this peptide dosage calculator recommend an amount?
No. It performs mass-to-volume conversion for the amount entered by the researcher. It does not choose an amount or research protocol.
Research supplies
Research Water
You’ll need this to reconstitute your peptides. 10mL, shipped from the US.