Kisspeptin Dosage Calculator and Chart: Kisspeptin-10 Protocol for Men and Women

Kisspeptin-10 is the last ten amino acids of kisspeptin, the hormone that sits at the very top of the reproductive chain: it tells the hypothalamus to release GnRH, GnRH tells the pituitary to release LH and FSH, and those two drive testosterone and estrogen. It is dosed in micrograms, and the protocols in circulation draw 100 to 200mcg at a time. Rhythm matters as much as the dose: in the human research, a pulse of kisspeptin works, and a signal that never switches off fades.

Simms Research sells Kisspeptin-10 in one size, a 10mg vial at $74, and the syringe figures in this guide are for that vial once 2mL of bacteriostatic water is in it.

Kisspeptin dosage calculator

Pick the water and the dose, and the calculator gives the units to draw on a U-100 insulin syringe. It opens on a 10mg vial with 2mL and a 100mcg dose: 2 units.

Kisspeptin dosage chart for the 10mg vial

100 to 200mcg per draw, once a day or every other day. With 2mL of bac water in the 10mg vial, a single unit on a U-100 insulin syringe holds 50mcg, so the whole common range spans 2 to 4 units:

Most protocols open at 100mcg, 2 units, and some stay there for the whole run. A second group spaces the doses out and draws more each time, 500mcg to 1mg every other day. Two to four units is a small draw, so a 0.3mL syringe, with one line per unit and half-unit marks on some brands, gives the cleanest reading. For a different vial size, more water or a dose between the rows, the peptide calculator returns the units.

Kisspeptin 10mg reconstitution

Add 2mL. At 5mg per mL, one unit carries 50mcg and ten units carry 500mcg, the strength the chart is built on. Kisspeptin-10 dissolves straight into plain bac water, so no acetic acid or other solvent is needed.

Aim the stream at the glass wall rather than the powder and swirl slowly; a 10mg cake of kisspeptin-10 is gone in about a minute and leaves a solution with no color and no haze. Mark the mixing day on the label, refrigerate it and use it up inside four weeks. Every step, for any vial, is set out in how to reconstitute peptides.

Simms Research’s Research Water, preserved with 0.9% benzyl alcohol, costs $20 for 10mL and covers five kisspeptin vials. What the benzyl alcohol adds over plain sterile water is explained in the bac water guide.

Kisspeptin-10 dosage in the human studies

Smaller, weighed per kilogram and given into a vein. The first study of kisspeptin-10 in men, published in 2011 in the Journal of Clinical Endocrinology and Metabolism, gave healthy volunteers single IV doses from 0.01 to 3µg per kilogram. LH rose quickly after every effective dose and peaked within 45 minutes. The strongest response came at 1µg/kg, which tripled LH in half an hour, and 3µg/kg drew a smaller rise than 1µg/kg did (George, Journal of Clinical Endocrinology and Metabolism, 2011): past a point, more kisspeptin bought less.

Scaled to an 80kg adult and read off the 2mL mix, the study doses look like this:

Dosing sites take the 100mcg draw straight from that 1µg/kg peak, rounded for an adult of about 100kg. The route differs: the dose-finding was done by vein, and community protocols give it under the skin, where it reaches the blood more slowly. The infusion row is the other extreme, a steady drip carrying far more peptide than any circulating protocol, and it is where the testosterone rise below came from.

Kisspeptin peptide benefits in research

Every benefit traces back to one action: kisspeptin raises LH and FSH by making the body release its own GnRH.

In fertility work, a single injection of kisspeptin-54 under the skin matured eggs in 53 women going through IVF, published in the Journal of Clinical Investigation in 2014; 92% went on to embryo transfer and 23% reached a clinical pregnancy. The same team then tested it in women at high risk of ovarian hyperstimulation, the complication the usual hCG trigger can set off. In women whose periods had stopped because the hypothalamus had gone quiet, kisspeptin-54 twice a week for eight weeks kept their reproductive hormones raised compared with saline.

The newest findings concern desire. In a 2023 JAMA Network Open trial of 32 men with low sexual desire, a 75-minute kisspeptin-54 infusion changed activity across the brain’s sexual-processing network and raised measured physical arousal by up to 56% more than placebo. A matching 2022 trial in 32 women with the same diagnosis found changes in how their brains processed sexual and attraction cues. Most of these studies used kisspeptin-54; the work on kisspeptin-10 itself centers on LH and testosterone.

Kisspeptin dosage for men and testosterone

100 to 200mcg, with no adjustment for body weight, and the studies do show testosterone rising, by way of LH. In the 2011 study, healthy men on a 22.5-hour drip of kisspeptin-10 at 4µg/kg an hour went from an average LH of 5.4 to 20.8 IU/L, and their testosterone climbed from 16.6 to 24.0 nmol/L, about 45%, while staying inside the normal range. A gentler drip, 1.5µg/kg an hour, made the natural LH pulses both more frequent and larger.

A 2013 follow-up in Clinical Endocrinology ran the same test in men with type 2 diabetes and low testosterone. A single 0.3µg/kg dose lifted their LH about as much as in healthy men, and an 11-hour drip at 4µg/kg an hour took testosterone from 8.5 to 11.4 nmol/L (George, Clinical Endocrinology, 2013).

Every one of these results depends on a working pituitary and testes: kisspeptin adds no hormone of its own, so the rise is only as large as the testes’ answer to LH. And a 100mcg pulse lifts LH for a short window, where the drips held it up for hours.

Kisspeptin dosage for women

Protocols in circulation give women the same 100 to 200mcg draws as men, but in the studies the day of the menstrual cycle mattered more than the dose. The 2011 study that compared men and women found that kisspeptin-10 changed nothing in the follicular phase, the first half of the cycle: not as an IV dose of up to 10nmol/kg, not under the skin at up to 32nmol/kg (around 2.7mg for a 65kg woman), and not as a drip. In the days before ovulation, the same IV dose raised both LH and FSH. Men in that study responded to doses as low as 0.3nmol/kg.

A 2012 study in Human Reproduction then gave 0.3µg/kg to three groups. It found a small LH rise early in the cycle, a response more than twice as large after menopause, the only group where FSH rose too, and no response in women taking the combined pill. Kisspeptin-54 follows the same calendar: in a 2007 study, one fixed dose drew its largest LH response before ovulation and its smallest in the follicular phase.

So research protocols for women keep the chart’s draws, and the hormonal background, not the dose, sets the result.

Kisspeptin cycle length and pulsing

A run of four to six weeks followed by roughly a month without it is the pattern most guides share, with 8 to 12 weeks at the long end. The spacing between draws matters more than the length of the run, because the main finding on repeated kisspeptin is that a constant signal fades.

Women with hypothalamic amenorrhea given kisspeptin-54 twice a day showed it clearly in a 2009 study: the first injection raised LH by an average of 24.0 IU/L, and by day 14 the same injection raised it by 2.5. A 2010 follow-up found the FSH response nearly gone by day 2, while twice-weekly injections desensitized only partly and still kept hormones raised after eight weeks. In juvenile male monkeys, a nonstop kisspeptin-10 infusion lifted LH for about three hours before it dropped, and in adult male rats a pump of kisspeptin-54 raised LH and testosterone on day 1, lost the effect by day 2, and left the testes lighter after 13 days.

Healthy men are the exception at short range: in the 2011 study, LH was as high in the last 90 minutes of a 22.5-hour kisspeptin-10 drip as in the first. That is why circulating protocols give single pulses, once a day or every other day, never anything continuous, and why the every-other-day camp spaces them further. No study has compared those two rhythms for kisspeptin-10 under the skin; the closest evidence is kisspeptin-54, where twice a day faded and twice a week held.

How long a kisspeptin 10mg vial lasts

Four weeks on almost every protocol. A mixed vial is good for 28 days, and at these small draws the calendar ends it long before the powder runs out:

A 10mg vial holds 100 draws of 100mcg, which is where the 100-day and 200-day supplies quoted on dosing sites come from, but those figures leave out the 28-day limit. A block of 8 to 12 weeks means mixing a fresh vial every four weeks.

Kisspeptin-10 vs kisspeptin-54

Same receptor, same job, different length and staying power. Kisspeptin-54 is the main form circulating in human blood; kisspeptin-10 is its last ten amino acids, the shortest piece that still fully switches on the kisspeptin receptor. Kisspeptin-54 lasts about 28 minutes in the blood (Dhillo, Journal of Clinical Endocrinology and Metabolism, 2005), seven times longer than kisspeptin-10.

In a 2015 head-to-head study in healthy men, three-hour IV infusions of the two at the same molar dose produced similar LH responses, both well below what GnRH itself achieved. The trial doses of kisspeptin-54, given in nmol per kilogram, don’t carry over to kisspeptin-10: each kisspeptin-54 molecule weighs about four and a half times as much. The chart in this guide is for kisspeptin-10 only.

Kisspeptin side effects

In the human studies, none worth recording. The 2011 kisspeptin-10 study reported no adverse events, with blood pressure, heart rate, oxygen levels, liver and kidney function and blood counts steady throughout, and the eight-week twice-weekly kisspeptin-54 study saw no adverse effects either. Both desire trials found it well tolerated, the 2023 men’s trial with no side effects or adverse events reported and no meaningful change in blood pressure or heart rate.

Dosing sites list mild warmth or flushing soon after a draw, brief redness where it went in and the occasional passing headache. The effect the research does document is the fading response to constant exposure, which spacing handles.

Frequently asked questions

How long does it take for kisspeptin-10 to kick in?

Minutes. In the IV studies LH peaked within 45 minutes, and kisspeptin-10 itself clears from the blood with a half-life of about 4 minutes (Jayasena, Journal of Clinical Endocrinology and Metabolism, 2011), so each draw acts as a short pulse. Testosterone follows LH over the next hours.

Is kisspeptin taken in the morning or at night?

Most protocols use the morning, when the body’s own testosterone runs highest, at the same time each day.

How do I mix a 5mg kisspeptin vial?

Many sellers stock 5mg vials. 1mL of bac water in a 5mg vial gives the same strength as 2mL in a 10mg vial, so the chart above reads unchanged.

What is the difference between kisspeptin and hCG?

hCG acts on the testes and ovaries directly, standing in for LH. Kisspeptin acts at the top of the chain and works only through the body’s own GnRH and LH, so it needs a responsive hypothalamus and pituitary, and each dose is a short pulse. In IVF research, kisspeptin-54 was tested as an alternative to the hCG trigger.

Is kisspeptin the same as PT-141?

No. Kisspeptin moves reproductive hormones; PT-141 acts on melanocortin receptors in the brain linked to desire and leaves those hormones alone. Its draws are in the PT-141 dosage chart.

How long does mixed kisspeptin last?

28 days at 36 to 46°F (2 to 8°C). Unmixed, the powder rides out room temperature in transit, and Simms Research ships it without ice packs; the peptide refrigeration guide covers powder and mixed vials.

Is kisspeptin FDA approved?

No. It remains under clinical study, and Simms Research sells kisspeptin-10 for laboratory research only.