Sermorelin Dosage Calculator and Chart: Bedtime Injection Protocol (2026)

Sermorelin is the first 29 amino acids of GHRH, the signal the hypothalamus sends to the pituitary to release growth hormone. It was a prescription drug, Geref, for children short on growth hormone until its maker stopped selling it in 2008, and it remains one of the most studied growth hormone peptides. Protocols measure it in micrograms and run it at night, and it clears the blood within minutes.

At Simms Research, Sermorelin comes in one size: 10mg of powder for $78. All the unit counts here are for that vial once it holds 2mL of bac water.

Sermorelin dosage calculator

Pick the water and the dose, and the calculator gives the units to draw on a U-100 insulin syringe. It opens on a 10mg vial with 2mL and a 300mcg dose: 6 units.

Sermorelin dosage chart

The usual nightly amount is 200 to 300mcg, which comes to 4 to 6 units. With 2mL of water in the vial, each single line on a U-100 insulin syringe carries 50mcg of sermorelin:

Protocols typically begin with a week or two at 100 to 200mcg before holding at 300mcg, and a minority step up to 500mcg after two months. At these volumes the half-size 0.3mL syringe is easier to read, since its markings run one unit apart. The studies behind the 1mg row are set out further down. Other vial sizes and other water volumes go through the peptide calculator.

How to reconstitute sermorelin 10mg

With 2mL of bacteriostatic water, which makes the solution 5mg per mL: half a milligram in 10 units, and the chart above applies as written. Some guides add 3mL or 5mL instead, which makes every unit weaker and the unit counts larger; the peptide calculator converts those mixes.

Geref’s own label set the method still used today: water aimed at the glass wall, then a slow circular swirl until no powder remains, never a shake. The result should look like plain water. Date the vial, refrigerate it and use it up within 28 days; the step-by-step version sits in how to reconstitute peptides.

Simms Research’s Research Water, $20 for 10mL, holds enough for five of these 2mL mixes. Why it carries benzyl alcohol, and why that matters for a vial opened every night for four weeks, is in the bac water guide.

Is 30 units of sermorelin too much?

For this vial at 2mL, yes. 30 units is 1.5mg, five times the usual 300mcg draw and well beyond the range protocols run on. A unit is only a volume, and what it holds depends on the mix.

The 30-unit figure comes from clinic sermorelin, which is far more dilute. A 3mg vial mixed with 3mL holds 1mg per mL, so 30 units is 300mcg, and one compounding pharmacy’s ready-mixed sermorelin at 0.9mg per mL is dosed at 33 units for the same 300mcg. The same 300mcg here is just 6 units. Unit counts copied from a clinic sheet or another vendor’s chart don’t carry over to this vial.

Sermorelin dosage for men and muscle growth

The same 200 to 300mcg a night; protocols don’t adjust the draw for sex or body weight. The published studies dosed far higher, and they are where the muscle evidence comes from:

On this vial that means 20 units for 1mg, 40 for 2mg, and about 16 for 10mcg per kg in an 80kg man. The muscle findings are modest and come from men aged 55 to 76. The 2mg study found that one injection a night raised growth hormone without moving IGF-1, and its authors judged several doses a day more effective. Protocols in circulation still keep to one smaller draw at bedtime, and a few aimed at body composition run 500mcg, 10 units.

Sermorelin at bedtime, on an empty stomach

At bedtime, two hours or more after the last meal. The body releases its largest pulse of growth hormone soon after falling asleep, and a bedtime draw adds to that pulse instead of creating one at an odd hour. Geref was given once a day at bedtime, and the 16-week study of older adults injected at 9pm: growth hormone rose within 10 minutes and stayed up for about two hours.

The empty stomach matters because a meal, sugar especially, blunts the pituitary’s answer to a growth hormone signal. Protocols leave two to three hours after dinner and skip food for about 30 minutes after the draw. The research and a meal-timing table are in the CJC-1295 and ipamorelin dosage guide. A morning draw still works, but misses the night pulse; schedules that add one, like the 1992 twice-a-day study, keep the bedtime draw as well.

Sermorelin 5 days on, 2 days off

Five nights on and two off is the most common weekly pattern, often Sunday to Thursday with Friday and Saturday free. At 300mcg a night it comes to 1.5mg a week:

The two nights off are a protocol habit meant to keep the pituitary responsive and stretch each vial; no study has compared them with nightly use. Every study above ran nightly without breaks, and in the 16-week one the growth hormone response held to the end, though IGF-1 drifted back toward its starting level by the final month. Blocks run three to six months, or eight to twelve weeks on and four to eight off. The Geref trial ran a full year.

How long a 10mg bottle of sermorelin lasts

Four weeks at the usual doses. A 10mg vial holds 33 draws of 300mcg, but a mixed vial keeps for 28 days, so on most schedules the calendar ends it before the powder runs out:

At 200 to 300mcg a vial is retired on day 28 with some sermorelin still in it, and a fresh one is mixed for the next four weeks. Only nightly 500mcg empties it first. Unmixed vials keep in the fridge until needed, as the peptide refrigeration guide explains.

Sermorelin before and after: the timeline

Two weeks for IGF-1 to rise, about four months for changes in the body (Khorram, Journal of Clinical Endocrinology and Metabolism, 1997). The studies above line up like this:

Body weight didn’t move in either adult study, so the before-and-after change these studies recorded is lean mass and skin rather than the scale. User reports name better sleep first, within a few weeks, though the 16-week study measured no change in sleep quality; they put body composition at three to six months.

Sermorelin side effects

Few, and mostly at the injection spot. In Geref’s clinical trials the most common side effect, in about one patient in six, was a local reaction where it went in: pain, swelling or redness. Three of the 350 patients stopped over it. Everything else on the label (headache, flushing, dizziness, trouble swallowing, hyperactivity, sleepiness and hives) appeared in fewer than 1 in 100.

The adult studies were just as quiet. The 6-week study reported no significant side effects, and the 16-week one only a temporary rise in blood fats that had settled by its end. Facial flushing is more common when sermorelin goes into a vein for pituitary testing than with a nightly draw under the skin.

Sermorelin vs tesamorelin, ipamorelin and CJC-1295

All four act on the same pituitary cells. They differ in which signal they copy and how long it lasts. Tesamorelin is the full 44-amino-acid GHRH with a small cap that shields it from breakdown, approved as Egrifta for abdominal fat in HIV and run at 1 to 2mg a day; its units are in the tesamorelin dosage guide. CJC-1295 without DAC is sermorelin’s own chain with four amino acids swapped, which stretches its half-life from about 11 minutes to about 30.

Ipamorelin copies ghrelin instead and works through a separate receptor, so it is paired with a GHRH peptide rather than swapped for one: at bedtime it runs at 100 to 200mcg beside sermorelin, each from its own vial, as the ipamorelin dosage chart lays out. The ready-made CJC-1295 and ipamorelin blend has its own water and units in the CJC-1295 and ipamorelin dosage guide.

Sermorelin purity and content by batch

Each published batch came in a little over the 10mg label. An independent ISO-certified lab ran three vials of the current batch separately on July 16, 2026:

RP-HPLC set the purity and LC-MS confirmed the molecule. The same panel cleared endotoxins, detected no heavy metals and found no microbial growth. May 2026’s batch, a single vial, read 10.61mg and 99.83% on HPLC alone, from before contaminant screening was added.

In practice the overage is small: with 10.64mg dissolved in 2mL, 6 units holds about 320mcg instead of 300mcg, and typing 10.64 into the calculator’s vial field corrects the units for this batch. Both certificates are posted in full under Sermorelin lab results, and how to read a peptide COA explains each line.

Frequently asked questions

How do you dose a 5mg vial of sermorelin?

The micrograms stay the same and the units change. A 5mg vial given 2mL holds 25mcg per unit, so 300mcg is 12 units. The calculator covers any other vial.

How many units of sermorelin per day?

4 to 6 units, 200 to 300mcg, once a night when the 10mg vial holds 2mL; opening weeks often use 2 to 4.

How long does sermorelin last in the body?

Its half-life is 11 to 12 minutes, and the growth hormone pulse it triggers is over in about two hours. IGF-1, which follows that pulse, stays raised far longer.

How long does mixed sermorelin last in the fridge?

28 days at 36–46°F (2–8°C). Unmixed, the powder tolerates weeks at room temperature, so it travels from Simms Research with no ice in the box.

Can sermorelin be taken as a nasal spray or by mouth?

Both are sold, but every study behind these doses injected it under the skin, and even that way only about 6% of a dose reaches the blood, according to Geref’s label. The units here are for the injected peptide.

Is sermorelin FDA approved?

It was, as Geref, from 1990 for pituitary testing and from 1997 for children short on growth hormone. Its maker discontinued it in 2008, and in 2013 the FDA confirmed the withdrawal was not for safety or effectiveness. Today’s research vials, Simms Research’s included, are for laboratory use only.