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AOD-9604 Dosage Chart and Calculator: Daily Fat Loss Protocol (2026)
AOD-9604 dosage for the 10mg vial: the daily chart in units with 2mL of bac water, morning timing, cycle length, how long a vial lasts and what the human trials found.
AOD-9604 is the tail end of human growth hormone, residues 177 to 191 with a tyrosine added at the front, built at Monash University in Australia as an anti-obesity drug. It carries the hormone’s fat-releasing signal without touching IGF-1 or blood sugar. Research protocols give it once a day, most often 300mcg first thing in the morning, which from a 10mg vial is 6 units on the syringe.
Simms Research sells AOD-9604 as a 10mg vial at $64, and the syringe numbers in this guide all come from that vial after 2mL of bac water goes in.
AOD-9604 dosage calculator
Pick the water and the dose, and the calculator gives the units to draw on a U-100 insulin syringe. It opens on a 10mg vial with 2mL and a 300mcg dose: 6 units.
AOD-9604 dosage chart
250 to 500mcg a day, in one draw. Once 2mL of bac water is in the 10mg vial, each 10-unit mark on a U-100 insulin syringe holds 500mcg:
| Daily dose | Units on a U-100 syringe | Doses in a 10mg vial | Where it sits |
|---|---|---|---|
| 250mcg | 5 units | 40 | Starting dose |
| 300mcg | 6 units | 33 | The usual daily draw |
| 500mcg | 10 units | 20 | Top of the usual range |
| 1mg | 20 units | 10 | Highest dose in circulation |
300mcg, 6 units, is where most protocols settle. Every row fits a small insulin syringe, and the 0.3mL size, marked in single units, makes a 5 or 6 unit draw easy to read. The 1mg row exists because the developer’s trials used 1mg pills; injected protocols rarely go that high.
AOD-9604 reconstitution: how much bac water
2mL for the 10mg vial. At that strength 10 units hold 500mcg and every dose in the chart lands on a whole line. The water to use is bacteriostatic, sterile water preserved with benzyl alcohol (0.9%), so a vial pierced every morning for four weeks stays free of growth.
Aim the stream at the glass, not the powder, and let it run down slowly. Then roll the vial between your palms until the cake has gone, with no shaking. Once mixed it lives in the fridge, between 2 and 8°C, and is finished inside 28 days; the reconstitution guide covers the full routine for any vial.
Simms Research’s 10mL bottle of Research Water, $20, is enough for five AOD-9604 vials, and the bac water guide explains why the preserved kind matters and how to tell the real thing.
Vials of 2mg and 5mg are common elsewhere, and some guides mix with less water. Any vial, amount of water or dose outside the chart goes into the peptide calculator, which gives back the units.
Why AOD-9604 turns cloudy after reconstitution
Air, nearly always. A fast stream or a shake drives fine bubbles through the liquid, and for the first few minutes the vial looks milky. Left upright in the fridge it clears, and properly mixed AOD-9604 is as clear and colorless as water.
The cake can also clump into a soft gel when water hits it hard, a complaint that comes up more often with AOD-9604 than with most peptides; a slow trickle down the glass avoids it, and a gentle roll dissolves what has formed. Haze that lasts after a rest, specks that never dissolve or strings in the liquid rule the vial out. The hub shows what a mixed vial should look like.
AOD-9604 dosage per day and for beginners
A first run opens at 250mcg a day, 5 units, and most protocols step up to 300mcg after the first week or two. 500mcg, 10 units, is the top of the usual daily range, taken in one draw or split into two. None of them adjust the amount for body weight or sex.
That injected range comes from the protocols in circulation, because no trial gave AOD-9604 as a daily shot under the skin. The developer’s human studies used single intravenous infusions of 25 to 400mcg per kilogram, then capsules and tablets from 0.25 to 54mg (Stier, Journal of Endocrinology and Metabolism, 2013). The two long trials gave a daily pill for 12 and 24 weeks, and 1mg a day ran in both: it was the best performer in the 12-week trial.
AOD-9604 morning or night
Morning, before food. Protocols draw it on waking and wait 30 to 60 minutes before breakfast, or take it just ahead of a workout done fasted. The thinking rests on insulin: fat cells release fat most freely when insulin is low, as it is after a night without food, and a meal shuts that release down.
A night dose follows the same rule, at least four hours after the last meal. Protocols that split 500mcg into two draws put the first on waking and the second there.
How to use AOD-9604: protocol and cycle length
Eight to twelve weeks of daily draws, then four to eight weeks off. After the opening week at 250mcg, a typical run holds at 300mcg and moves to 500mcg in the second month only if the first showed little.
The break is a habit borrowed from other peptides more than a finding. The developer dosed people every day for 12 and 24 weeks straight, checked their blood for antibodies against the peptide along the way and found none, while IGF-1 stayed flat throughout. Nothing in that record points to the effect fading with use; the pause is a point to measure progress before the next block.
How long one AOD-9604 10mg vial lasts
Four weeks at the usual doses, less above them. A mixed vial keeps for 28 days in the fridge, and that, not the powder, sets the limit at 250 and 300mcg:
| Daily dose | One vial covers | Vials for 8 weeks | Vials for 12 weeks |
|---|---|---|---|
| 250mcg | 28 days, with 12 doses left over | 2 | 3 |
| 300mcg | 28 days, with 5 doses left over | 2 | 3 |
| 500mcg | 20 days | 3 | 5 |
| 1mg | 10 days | 6 | 9 |
At the usual doses the plan is one vial for each four-week block, mixed fresh at the start of it.
AOD-9604 benefits in the research
Three findings stand up. In obese mice, 14 days of AOD-9604 cut weight gain, raised fat burning and pushed up glycerol in the blood, the marker of fat being broken down, and in obese rats 19 days of oral dosing held weight gain to less than half that of untreated animals. Unlike growth hormone, it did this without raising blood sugar or binding the growth hormone receptor (Heffernan, International Journal of Obesity and Related Metabolic Disorders, 2001).
In people, six controlled trials measured the effects growth hormone is known for and found none of them: IGF-1, fasting glucose, insulin and glucose tolerance held steady at every dose (Stier, Journal of Endocrinology and Metabolism, 2013). The third finding is outside fat metabolism. In a rabbit model of knee osteoarthritis, weekly 0.25mg injections into the joint improved cartilage repair, and worked better still alongside hyaluronic acid.
AOD-9604 before and after: what the trials showed
Modest in people. The 12-week trial gave 300 adults with obesity a daily pill of 1, 5, 10, 20 or 30mg. The 1mg group lost the most, just under 3kg on average, about 2kg more than placebo, and the higher doses did no better. A 24-week trial in 502 adults then tested 0.25, 0.5 and 1mg a day and found no weight loss beyond placebo, and the developer closed the obesity program in February 2007.
AOD-9604 also leaves appetite alone; the animal work traced its effect to fat tissue itself. So a before and after on AOD-9604 is read on the tape measure and body-fat readings across 8 to 12 weeks, not in the fast scale drops of a GLP-1 class peptide.
AOD-9604 side effects
No different from placebo. That is the verdict of the 2013 safety review in the Journal of Endocrinology and Metabolism, which pooled six controlled trials and 893 adults. Headache was the top complaint study after study, at much the same rate on placebo, followed by mild stomach upset such as loose stools. In the 24-week trial, fewer people on the lowest dose reported any complaint at all than on placebo, 75.6% against 83.2%.
The trials found no antibodies against the peptide and no rise in IGF-1 or blood sugar, the effects that keep full growth hormone out of long-term use. Across all six, the review found no withdrawal or serious event caused by the peptide. The only dose tied to more headaches and loose stools was a 54mg oral capsule, far above anything in circulation. People injecting it under the skin also report a brief sting or redness where it goes in.
AOD-9604 vs tesamorelin
They reach fat from opposite ends. Tesamorelin is a growth-hormone-releasing hormone analog: it has the pituitary release more growth hormone, which lifts IGF-1, and at 2mg a day it cut visceral abdominal fat in the trials behind its approval (Egrifta label, FDA, 2010). AOD-9604 bypasses the pituitary, leaves IGF-1 where it was and carries only the fat-releasing end of the hormone. Run together, each keeps its own vial and dose, and the tesamorelin dosage guide has tesamorelin’s units for both vial sizes.
AOD-9604 vs MOTS-c is the other common comparison. MOTS-c is a mitochondrial peptide dosed in milligrams a few times a week, set out in the MOTS-c dosage chart.
Frequently asked questions
How many units of AOD-9604 is a dose?
6 units for the usual 300mcg, with the daily range running from 5 to 10 units once the 10mg vial is mixed at 2mL. Units only mean something next to the mix: the same 6 units from a 5mg vial with 2mL hold half as much.
How much BAC water for AOD-9604?
It takes 2mL, which makes 10 units 500mcg, 6 units 300mcg and 5 units 250mcg.
Does AOD-9604 raise IGF-1 like growth hormone?
No. Every human trial measured IGF-1 and none saw it move, at any dose or route. It is a fragment of growth hormone, not the hormone, and in cell tests it did not bind the growth hormone receptor.
Is AOD-9604 the same as HGH fragment 176-191?
They describe the same stretch at the end of growth hormone. AOD-9604 is that stretch with its first amino acid, a phenylalanine, swapped for a tyrosine, and the two names are often used for the same powder.
Can AOD-9604 be taken as a pill or nasal spray?
It was built as a pill: the two long trials used daily capsules and tablets, with the modest results above. Sprays and troches are sold as well; the chart here covers the injectable 10mg vial only.
Can AOD-9604 be stacked with CJC-1295 and ipamorelin?
Yes, drawn separately at their usual doses. The pair raises growth hormone itself while AOD-9604 works on fat only; the CJC-1295 / Ipamorelin dosage guide has the units for the blended vial.
Does AOD-9604 need to be refrigerated?
Once mixed, yes, and used within 28 days. The dry powder stays stable for weeks outside the fridge, so it travels without cold packs; the peptide storage guide covers both stages.
Is AOD-9604 FDA approved?
No. It never reached the market as a drug, and Simms Research sells it for laboratory research only.